|
KMID : 1123920220360040125
|
|
Korean Journal of Oriental Physiology and Pathology
2022 Volume.36 No. 4 p.125 ~ p.129
|
|
|
In vitro Anti-oxidant and Anti-inflammatory Effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) Mixed Formula
|
|
Heo Seok-Mo
Yang Jin-Won
Kim Jong-Lae
Park Mi-Ryeong
Kim Tae-Gi
Ku Sae-Kwang
Park Soo-Jin
|
|
|
Abstract
|
|
|
|
The purpose of study is to evaluate in vitro anti-oxidant and anti-inflammatory effects of Moringa Folium and Eucommiae Cortex 2:1 (g/g) mixtures (MEMix). HaCaT and human normal dermal fibroblast were treated with 0.01-1 mg/mL of MEMix to monitor cytotoxicity. Radical scavenging activities of MEMix were examined by DPPH assay. To explore anti-inflammatory effect, Raw 264.7 cells were pretreated with MEMix for 1h and subsequently exposed to LPS for 18h. NO release and cytotoxicity of Raw 264.7 cells were measured by adding Griess and MTT reagents, respectively. TNF-α, IL-1β, IL-6, and PGE2 productions were examined by ELISA. Immunoblot analysis was conducted to examine COX-2 expression in MEMix pretreated Raw 264.7 cells. Up to 1 mg/mL concentration, treatment of MEMix for 24 h did not affect normal dermal fibroblast viability and significantly reduced cell viability of HaCaT cells with no concentration dependency. MEMix increased DPPH radical scavenging activity with concentration dependency. Radical scavenging activities by 1 mg/mL of MEMix was comparable with 30 μM of trolox. Pretreatment of MEMix did not change the reduction of Raw 264.7 cell viability. Exposure of LPS in Raw 264.7 cells significantly increased NO, TNF-α, IL-1β, IL-6, and PGE2 productions, and MEMix pretreatment attenuated these productions by LPS concentration dependently. However, pretreatment with MEMix did not change COX-2 expression by LPS in Raw 264.7 cells. MEMix showed in vitro anti-oxidant and anti-inflammatory activities. MEMix would be useful candidate agent against inflammation.
|
|
|
KEYWORD
|
|
|
Moringa Folium, Eucommiae Cortex, Moringa Folium and Eucommiae Cortex 2:1 (g/g) mixtures, DPPH radical scavenging activity, Anti-inflammatory effect
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|